Composite

Part:BBa_K3890000:Design

Designed by: Beatriz Toledo Akiti   Group: iGEM21_USP-Brazil   (2021-09-30)


Pollen expressed CYP6G1 and GUS reporter circuit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2088
    Illegal SapI site found at 1918


Design Notes

The CYP codons are optimized for tomato expression


Source

NCBI Solanum lycopersicum genome NCBI Drosophila melanogaster genome SUN H, Zhou N, Wang H, Huang D, Lang Z. Processing and targeting of proteins derived from polyprotein with 2A and LP4/2A as peptide linkers in a maize expression system. PLoS One. 2017 Mar 30;12(3):e0174804. doi: 10.1371/journal.pone.0174804. PMID: 28358924; PMCID: PMC5373624.

References